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Gretchen Halpert <[log in to unmask]>
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SciArt-L Discussion List-for Natural Science Illustration- <[log in to unmask]>
Fri, 23 May 1997 13:16:01 -0400
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To Kathy Evans looking for methods of cutting cross sections of grass stems...

Hi Kathy.  What level of observation are you looking for?  Detailed
histology?  Dissecting or compound scope photography?  ( I assume not at
the SEM level). You can try freezing the tissue, but you will lose
morphology (fix the tissue, embed it in a mounting media like OCT and
section it on a cryostat).  I would reccomend fixing it, embedding it in
paraffin and cutting it on a microtome.  There are fixatives specific for
plant tissues that penetrate the cellulose-don't know any off the top of my
head, but probably a formalin/alcolhol/acetic acid combination.  I can find
out if you need me to.  Of course, you can't really do this at home, but
most universities and hospitals have a histology department with the
solutions and cutting equipment (microtome) you need.You can also stain the
section with nifty colored stains to differentiate between cell types. Good

Gretchen Halpert