Would the boiling water denature the DNA that the person is trying to
collect? - Barry

On Wed, Nov 29, 2017 at 11:43 AM, Derek Woller <[log in to unmask]> wrote:

> Floyd's suggestion sounds more exciting, but here's another that I use on
> Orthoptera specimens with great success: buy a very cheap electric water
> boiler (often used for tea) with a wide mouth, like this one:
> B004YTW58S/ref=sr_1_4?s=kitchen&ie=UTF8&qid=1511973780&sr=1-4&keywords=
> electric+water+boiler
> and then simply hold the specimen over the mouth once the water is boiling
> to loosen the specimen or give it a quick dip (too long and coloration
> alteration may occur). Then, I just hold the pin in one hand and use
> forceps in the other to slide the specimen off. Granted, I have not tried
> this methodology with non-orthopterans, but I don't see why it wouldn't
> work for other taxa. I also hear a drop of ammonia around the pin might
> work, but I have not tested that method since this one works just fine for
> me.
> Good luck!
> *Derek A. Woller, **Ph.D.*
> *Entomologist, *Rangeland Grasshopper and Mormon Cricket Management Team,
> USDA: APHIS, PPQ, S&T, CPHST Phoenix Lab
> 3645 East Wier Ave., Phoenix, AZ, 85040
> <,+Phoenix,+AZ,+85040&entry=gmail&source=g>
> Office: 602-431-3246 <(602)%20431-3246>
> Cell: 480-490-6454 <(480)%20490-6454>
> Fax: 602-431-3258 <(602)%20431-3258>
> [log in to unmask]
> ppq-program-overview/cphst/ct_dspmsl
> Proud to be an explorer of the final biological frontier: entomology.
> On Wed, Nov 29, 2017 at 9:13 AM, Michael Wilson <
> [log in to unmask]> wrote:
>> Dear Colleagues
>> Does anyone have a good method of removing a (beetle) specimen from a pin
>> without the use of a relaxing box?  A student wishes to place dried
>> specimens in a solution which will extract the body contents for DNA
>> extraction.  But needs to remove the pin first.
>> I have read about techniques that can heat a pin using a battery system
>> allowing the pin to be removed- but have not seen one in action!  Someone
>> else has suggested that a drop of 'wood naptha' at the base of the pin
>> would relax the specimen locally and allow the pin to be removed.
>> Any suggestions welcomed
>> Thanks in advance
>> Mike Wilson
>> Dr Michael R Wilson
>> Entomology Section
>> Dept of Natural Sciences
>> National Museum of Wales
>> Cardiff, CF10 3NP, UK
>> Tel: +44 2920 573263
>> email: [log in to unmask]
>> Rydym yn croesawu gohebiaeth yn Gymraeg a Saesneg, ac yn sicrhau y byddwn
>> yn cyfathrebu â chi yn eich iaith ddewisol, boed yn Gymraeg, Saesneg neu’r
>> ddwy, dim ond i chi ein hysbysu. Ni fydd gohebu yn Gymraeg yn peri oedi.
>> Mae pob neges e-bost a anfonir at neu gan Amgueddfa Cymru yn cael ei
>> sganio gan systemau diogelwch awtomatig. Sganiwyd y neges hon am firysau
>> cyn ei hanfon, ond dylech hefyd wirio bod y neges, a phob atodiad ynddi, yn
>> rhydd o firysau cyn ei defnyddio. Nid ydym yn derbyn cyfrifoldeb am unrhyw
>> golled neu ddifrod o ganlyniad i agor y neges neu unrhyw atodiadau. Gall y
>> neges hon ac unrhyw ffeiliau a atodir ynddi gynnwys gwybodaeth gyfrinachol
>> a fwriadwyd ar gyfer y derbynnydd yn unig. Os ydych chi wedi derbyn y neges
>> trwy gamgymeriad, hysbyswch ni a dileu’r neges. Safbwyntiau personol yr
>> awdur a fynegir yn y neges hon, ac nid ydynt o reidrwydd yn cynrychioli
>> safbwyntiau Amgueddfa Cymru. Nid ydym yn derbyn cyfrifoldeb am unrhyw
>> wallau, llygredd neu esgeulustod a allai godi wrth drosglwyddo'r neges hon.
>> We welcome correspondence in Welsh and English, and we will ensure that
>> we communicate with you in the language of your choice, whether that’s
>> English, Welsh or both if you let us know which you prefer. Corresponding
>> in Welsh will not lead to any delay.
>> E-mail to and from Amgueddfa Cymru is scanned by automated security
>> systems. This message was scanned for viruses before transmission, but you
>> should also satisfy yourself that the message, and all attachments, are
>> virus-free before use. We can accept no responsibility for any loss or
>> damage that might arise from opening the message or any attachments. This
>> message and any files transmitted with it may contain confidential
>> information intended only for the recipient. If you receive the message by
>> mistake please inform us and delete it. The views expressed in this message
>> are the personal views of the author and may not necessarily represent
>> those of Amgueddfa Cymru. We accept no liability for any errors, corruption
>> or omissions that might arise in transmission of this message.
>> --
>>  Scanned by FuseMail.

-So many mites, so little time!

Barry M. OConnor
Professor  & Curator
Department of Ecology & Evolutionary Biology
University of Michigan                  phone: 734-763-4354
1109 Geddes Ave.                          fax: 734-763-4080
Ann Arbor, MI 48109-1079          e-mail: [log in to unmask]