Derek A. Woller, Ph.D.
Entomologist, Rangeland Grasshopper and Mormon Cricket Management Team,
USDA: APHIS, PPQ, S&T, CPHST Phoenix Lab
3645 East Wier Ave., Phoenix, AZ, 85040
Proud to be an explorer of the final biological frontier: entomology.
Would the boiling water denature the DNA that the person is trying to collect? - Barry--On Wed, Nov 29, 2017 at 11:43 AM, Derek Woller <[log in to unmask]> wrote:Floyd's suggestion sounds more exciting, but here's another that I use on Orthoptera specimens with great success: buy a very cheap electric water boiler (often used for tea) with a wide mouth, like this one:https://www.amazon.com/Proctor
-Silex-32oz-Hot-pot/dp/B004YTW 58S/ref=sr_1_4?s=kitchen&ie= UTF8&qid=1511973780&sr=1-4& keywords=electric+water+boilerand then simply hold the specimen over the mouth once the water is boiling to loosen the specimen or give it a quick dip (too long and coloration alteration may occur). Then, I just hold the pin in one hand and use forceps in the other to slide the specimen off. Granted, I have not tried this methodology with non-orthopterans, but I don't see why it wouldn't work for other taxa. I also hear a drop of ammonia around the pin might work, but I have not tested that method since this one works just fine for me.Good luck!
Proud to be an explorer of the final biological frontier: entomology.On Wed, Nov 29, 2017 at 9:13 AM, Michael Wilson <[log in to unmask]
Does anyone have a good method of removing a (beetle) specimen from a pin without the use of a relaxing box? A student wishes to place dried specimens in a solution which will extract the body contents for DNA extraction. But needs to remove the pin first.
I have read about techniques that can heat a pin using a battery system allowing the pin to be removed- but have not seen one in action! Someone else has suggested that a drop of 'wood naptha' at the base of the pin would relax the specimen locally and allow the pin to be removed.
Any suggestions welcomed
Thanks in advance
Dr Michael R Wilson
Dept of Natural Sciences
National Museum of Wales
Cardiff, CF10 3NP, UK
Tel: +44 2920 573263
email: [log in to unmask]
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